Illumina HiSeq 2000 sequencing; RNA sequencing of beech roots and ass... - SRA

ERX10644825: Illumina HiSeq 2000 sequencing; RNA sequencing of beech roots and associated fungi
4 ILLUMINA (Illumina HiSeq 2000) runs: 27.3M spots, 3.9G bases, 1.9Gb downloads

Design: RNA sequencing of beech roots and associated fungi

Submitted by: Forest Botany and Tree Physiology, University of Geottingen (Forest Botany and Tree Physiology, University of G)

Study: RNA sequencing of beech roots and associated fungi

PRJEB61297 • ERP146398 • All experiments • All runs

show Abstracthide Abstract

Roots were collected from young trees outplanted in three regions in Germany (Schorfheide, Swabian Alb, Hainich) and used for RNA extraction. In each region four plots were sampled. Two plants used from each plot. The RNA of the plants per plot was pooled. Four technical replicates were prepared.

Sample: S5_S9

SAMEA112896194 • ERS14902333 • All experiments • All runs

Organism: Fagus sylvatica

Library:

Name: S5_S9_s

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Per plant: harvest of fine roots (< 2mm diameter) in the field, froozen in liquid nitrogen and transport to the lab on dry ice. Storage at -80°C until RNA extraction environmental samples Total RNA was extracted using the protocol of Chang S, Puryear J, Cairney J (1993, A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116). The RNA of four samples was pooled. The cDNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt am Main, Germany) according to the manufactorer´s protocol

Experiment attributes:

Experimental Factor: environmental history: Schorfheide

Runs: 4 runs, 27.3M spots, 3.9G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
ERR11218698	6,918,546	993M	496.5Mb	2023-04-23
ERR11218699	6,624,540	951.1M	477.4Mb	2023-04-23
ERR11218700	7,053,389	1G	505.2Mb	2023-04-23
ERR11218701	6,716,963	964.8M	482.7Mb	2023-04-23

ID:: 27467276

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity